JRC GMO-Amplicons | EURL GMFF

Record ID:	MK441680.1
Database:	nt	Go to original record
Description:	Hemiboea sp. ZH-2019 voucher Q. Zhang 380851 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Hemiboea sp. ZH-2019
Original size:	969 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	14	562	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt cagagaaacc ccggaattaa gaaaaatggg
101   caatcctgag ccaaatcctg ttttctcaaa acaagggttc agagagcgaa
151   aaagaggata ggtgcagaga ctcaatggaa gctgttctaa caaatggagt
201   tgactgcgtt ggtagaggaa tcgttccatc gaaacttcag aaaggatgaa
251   ggataaacgt ctctattgaa tactatatca aatgatatat aatggcgcga
301   atctggggtt ttgtatatga aaaatggaag aattggtgtg aatttctttc
351   acattgatga aagaatcgaa tattcattga tcaaatcatt cactccatag
401   tccgatagat cttttaaaga acttcttaat cggacgagaa taaagataga
451   gtcccgttct acatgtcaat accggcaaca atgaaacttc tagtaagagg
501   aaaatccgtc gacttttaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details