JRC GMO-Amplicons | EURL GMFF

Record ID:	MK441678.1
Database:	nt	Go to original record
Description:	Hemiboea sp. ZH-2019 voucher Q. Zhang 380849 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Hemiboea sp. ZH-2019
Original size:	967 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	14	560	+	547 bp

Amplicon:

547 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    actactaagt gaagactttc agagaaaccc cggaattaag aaaaatgggc
101   aatctgagcc aaatcctgtt ttctcaaaac aagggttcag agagcgaaaa
151   agaggatagg tgcagagact caatggaagc tgttctaaca aatggagttg
201   actgcgttgg tagaggaatc gttccatcga aacttcagaa aggatgaagg
251   ataaacgtct ctattgaata ctatatcaaa tgatatataa tggcgcgaat
301   ctggggtttt gtatatgaaa aatggaagaa ttggtgtgaa tttctttcac
351   attgatgaaa gaatcgaata ttcattgatc aaatcattca ctccatagtc
401   cgatagatct tttaaagaac ttcttaatcg gacgagaata aagatagagt
451   cccgttctac atgtcaatac cggcaacaat gaaacttcta gtaagaggaa
501   aatccgtcga cttttaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	547	528	2.46e-08

Match details