JRC GMO-Amplicons | EURL GMFF

Record ID:	MK441676.1
Database:	nt	Go to original record
Description:	Hemiboea sp. ZH-2019 voucher Q. Zhang 380847 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Hemiboea sp. ZH-2019
Original size:	957 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	10	550	+	541 bp

Amplicon:

541 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt cagagaaacc ccggaattaa taaaaatggg
101   caatcctgag ccaaatcctg ttttctcaaa acaagggttc agagagcgaa
151   aaagaggata ggtgcagaga ctcaatggaa gctgttctaa caaatggagt
201   tgaccgcgtt ggtagcagaa ttgaaacttc agaaaggatg aaggataaac
251   gtctctattg aatactatat caaatgatat ataatggcgc gaatctgggg
301   ttttgtatat gaaaaatgga agaattggtg tgaatttctt tcacattgat
351   gaaagaatcg aatattcatt gatcaaatca ttcactccat agtccgatag
401   atcttttaaa gaacttctta atcggacgag aataaagata gagtcccgtt
451   ctacatgtca ataccggcaa caatgaaact tctagtaaga ggaaaatccg
501   tcgactttta aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.43e-08
primerB	100.0	20 bp	0	0	541	522	2.43e-08

Match details