JRC GMO-Amplicons | EURL GMFF

Record ID:	EF395420.1
Database:	nt	Go to original record
Description:	Tsuga canadensis voucher YU Havill 03-07 tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tsuga canadensis
Original size:	1451 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	467	1023	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaattcagg gaaccccggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagacaatgg tttctttcct
151   aggataggaa ggggataggt gcagagactc aatggaagct attctaacga
201   atgaatttca tttggggtcc aatactctat ttatagaacg ctttatttac
251   acctcgaaag tgggaatgtt gtataaaatc aaacaacact cgcgatcaga
301   acttgaatcg ttccaagcat tttatttgta agataaatgc cagatttgag
351   ttgaagtaat gatttgacat taagtaatcc aattatgaac tttctctact
401   ctagataggg agttgaatca gtttttggaa taaattattg gacgaggata
451   aagagagagt ccaattctac gtgtcaatgt caacaacaat gcaaattgca
501   gtaggaggaa aatccgttgg ctttatagac cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details