JRC GMO-Amplicons | EURL GMFF

Record ID:	EF395419.1
Database:	nt	Go to original record
Description:	Nothotsuga longibracteata voucher PE LuoZC-002 tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Nothotsuga longibracteata
Original size:	1404 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	474	1028	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaattcagg gaaccctggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagacaatgg tttctttcct
151   aggataggaa ggggataggt gcagagactc aatggaagct attctaacga
201   atgaatctca tttggggtcc aatactctat ttatagaacg ctttatttac
251   acctcgaaag tgggaatgtt ttataaaata aaaaaaaact tgcgatcaga
301   acttgaatcg ttccaagcat tttattcgta agataaatgc cagatttgag
351   ttgaagtaat tatttgacat taagtaatcc cattatgaac ttctctactc
401   tagatagggg gttgaattag tttttggaat aaatgattgg acgaggataa
451   agatagagtc caattctacg tgtcaatgtc aacaacaatg caaattgcag
501   taggaggaaa atccgttggc tttatagacc gtgaggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details