JRC GMO-Amplicons | EURL GMFF

Record ID:	EF395418.1
Database:	nt	Go to original record
Description:	Pseudolarix amabilis voucher A 187-94 tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Pseudolarix amabilis
Original size:	1437 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	468	1029	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaatccagg gaaccctggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagacaatgg tttctttcct
151   aggcctagga taggaagggg ataggtgcag agactcaatg gaagctattc
201   taacgaatga atctcatttg gggtccaata ctctatttat agaacgttct
251   atttacacct cgaaagtggg aatgttgtat aacataaaac aaaactcgcg
301   atcagaactt gaatcgttcc aagcatttta ttcgtaagat agatgccaga
351   ttcgagttga agtcatgatt ttacattaag taatccaatt atgaacttat
401   ctactctaga tagggagttg aatcagtttt tggaataaat gattggacga
451   ggataaagat agagtccaat tctacgtgtc aatgtcaaca acaatgcaaa
501   ttgcagtagg aggaaaatcc gttggcttta tagaccgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details