JRC GMO-Amplicons | EURL GMFF

Record ID:	MK726018.1
Database:	nt	Go to original record
Description:	Larrea tridentata chloroplast, complete genome.
Taxon:	Larrea tridentata
Original size:	135988 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45774	46374	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttcca ataaaaacaa attaagagtt
151   catcaataaa aaatagaatt caaaaaggat aggtgcagag actcaatgga
201   agctgttctc aaaaatggag ttgactgcat ttactgtagt caaggaatcc
251   ttacatcgag acttcagaaa agatgaagga aaaccctgta tacataataa
301   aaattatgga aataatatat ccaattatga atttaaatct attttttttt
351   ttcaatttta tatttttcaa aatatgaaaa gaatttttga aatcaattga
401   aaattcaaga aataataaaa tagtatttga tccaattatt gactccaaag
451   tctgatttat cttttgaaaa aattgatgaa tccgatgaga ataaagatag
501   agtcccattc gataatgtca atattgacaa caatgaaatt tatattgaga
551   ggaaaatccg ttgattttat aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details