JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141667.1
Database:	nt	Go to original record
Description:	Galopina tomentosa isolate cX80 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF(GAA)) gene, partial sequence; chloroplast.
Taxon:	Galopina tomentosa
Original size:	1709 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	734	1320	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa aagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg gctgcggtag tcgagaagtc tttccatcaa aaattcataa
251   agtataagta ttaaggatga agtgaaggat aaacgtatat acatacgtag
301   tgaatagtat attaaatgag taatgataac ccgaatgaat ctttattttt
351   tctataaaaa acgaaagaat tgttcttaat agattccacg tttagaaaag
401   aatcgaatat tcattgatca aatgattcac tccatagtct gatagatcct
451   ttcacgaact gattaatcgg acgagaataa agatagagtc ccattctaca
501   tgtcaatgcc ggcaacaatg aaatttatag ttaagaggaa aatccgtcga
551   cttttaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details