JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141664.1
Database:	nt	Go to original record
Description:	Galopina aspera isolate cX98 tRNA-Thr (trnT(UGU)) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL(UAA)) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF(GAA)) gene, partial sequence; chloroplast.
Taxon:	Galopina aspera
Original size:	1716 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	747	1334	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt ggctgcggta gtcgagaagt ctttccatca aaaattcata
251   aagtataagt attaaggatg aagtgaagga taaacgtata tacatacgta
301   gtgaatagta tattaaatga gtaatgataa cccgaatgaa tctttatttt
351   ttctataaaa aacgaaagaa ttgttcttaa ttgattccac gtttagaaaa
401   gaatcgaata ttcattgatc aaatgattca ctccacagtc tgatagatcc
451   tttcactaac tgattaatcg gacgagaata aagatagagt cccattctac
501   atgtcaatgc cggcaacaat gaaatttata gttaagagga aaatccgtcg
551   acttttaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details