JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141642.1
Database:	nt	Go to original record
Description:	Anthospermum welwitschii isolate cX95 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF(GAA)) gene, partial sequence; chloroplast.
Taxon:	Anthospermum welwitschii
Original size:	1707 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	756	1343	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa agggaaaggg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggctgc ggtagtcaag aagtctttcc atcaaaaatt
251   aaactataag tattaaggat gaagtgaagg ataaacgtat atacatacgt
301   agtcaatagt atattaaatg agtaatgata acccgaatga atctttattt
351   ttctataaaa aaataaagaa ttgttcttaa tagattccac gtttagaaaa
401   gaatcgaata ttcattgatc aaatgattca ctccatagtc tgatagatcc
451   tttcacgaac tgattaatcg gacgagaata aagatagagt cccattctac
501   atgtcaatgt cggcaacaat gaaatttata gttaagagga aaatccgtcg
551   acttttaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details