JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141641.1
Database:	nt	Go to original record
Description:	Anthospermum sp. Bremer et al. 4351 isolate aL84 tRNA-Thr (trnT(UGU)) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL(UAA)) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF(GAA)) gene, partial sequence; chloroplast.
Taxon:	Anthospermum sp. Bremer et al. 4351
Original size:	1724 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	760	1349	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa agggaaaggg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggctgc ggtagtcaag aagtctttcc atcaaaaatt
251   cataaactat aagtattaag gatgaagtga aggataaacg tatatacata
301   cgtagtcaat agtatattaa atgagtaatg ataacccgaa tgaatcttta
351   tttttctata aaaaataaag aattgttctt aatagattcc acgtttagaa
401   aagaatcgaa tattcattga tcaaatgatt cactccatag tctgatagat
451   cctttcacga actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat gtcggcaaca atgaaattta tagttaagag gaaaatccgt
551   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details