JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141637.1
Database:	nt	Go to original record
Description:	Anthospermum galioides subsp. reflexifolium isolate aL82 tRNA-Thr (trnT(UGU)) gene, partial sequence; trnT-trnL intergenic spacer and tRNA-Leu (trnL(UAA)) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Anthospermum galioides subsp. reflexifolium
Original size:	1674 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	787	1377	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa agggaaaggg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggctgc ggtagtcaag aagtctttcc atcaaaaatt
251   cataaactat aagtattaag gatgaagtga aggataaacg tatatacata
301   cgtagtcaat agtatattaa atgagtaatg ataacccgaa tgaatcttta
351   tttttctata aaaaaataaa gaattgttct taatagattc cacgtttaga
401   aaagaatcga atattcattg atcaaatcat tcactccata gtctgataga
451   tcctttcacg aactgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa tgtcggcaac aatgaaattt atagttaaga ggaaaatccg
551   tcgactttta aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details