JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141619.1
Database:	nt	Go to original record
Description:	Anthospermum palustre isolate cY73 tRNA-Thr (trnT(UGU)) gene, partial sequence; trnT-trnL intergenic spacer and tRNA-Leu (trnL(UAA)) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Anthospermum palustre
Original size:	1663 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	739	1323	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttgg ctgcggtagt caagaagtct ttccatcaaa aattcataaa
251   ctataagtat taaggatgaa gtgaaggata aacgtatata catacgtagt
301   caatagtata ttaaatgagt aatgataacc cgaatgaatc tttatttttc
351   tataaaaaaa taaagaattg ttcttaatag attccacgtt tagaaaagaa
401   tcgaatattc attgatcaaa tgattcactc catagtctga tagatccttt
451   cacgaacgga ttaatcggac gagaataaag atagagtccc attctacatg
501   tcaatgtcgg caacaatgaa atttatagtt aagaggaaaa tccgtcgact
551   tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details