JRC GMO-Amplicons | EURL GMFF

Record ID:	MK141606.1
Database:	nt	Go to original record
Description:	Anthospermum bergianum isolate aL49 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF(GAA)) gene, partial sequence; chloroplast.
Taxon:	Anthospermum bergianum
Original size:	1524 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	542	1134	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa agggaaaggg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggctgc gcggtagtca agaagtcttt ccatcaaaaa
251   ttcataaact ataagtatta aggatgaagt gaaggataaa cgtatataca
301   tacgtagtca atagtatatt aaatgagtaa tgataacccg aatgaatctt
351   tatttttcta taaaaaaata aagaattgtt cttaatagat tccacgttta
401   gaaaagaatc gaatattcat tgatcaaatg attcactcca tagtctgata
451   gatcctttca cgaactgatt aatcggacga gaataaagat agagtcccat
501   tctacatgtc aatgtcggca acaatgaaat ttatagttaa gaggaaaatc
551   cgtcgacttt taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details