JRC GMO-Amplicons | EURL GMFF

Record ID:	MH165324.1
Database:	nt	Go to original record
Description:	Plantago ovata chloroplast, partial genome.
Taxon:	Plantago ovata
Original size:	162116 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45609	46191	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactagg tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtctt ctcaaaatca aaataaaggt
151   tcagaaagcg agaaaaaggg ataggtgcag agactcgaag gaagctgttc
201   taaaacaaat ggagttgact gcgttggtag ataaattttt tcatcgaaac
251   ttcagaaagg atgaaggata aacgtatcta ttctattgaa tactaaaata
301   tctcaaaaaa tgaatgacgg ccctagtcag tatctgtatt ttttttagat
351   gaaaaaatgg aagaattggt gtgaattgat tccacattca agaatgaatc
401   gaatattcat tcatcaaatc accccactcc atagtctgat agttcttttt
451   aaaaagaatt ggattggacg agaataaaga tagagtcccg ttctacatgt
501   caatatcgac agcaatgaaa tttatagtaa taggaaaatc cgtcgacttt
551   taaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details