JRC GMO-Amplicons | EURL GMFF

Record ID:	MH095698.1
Database:	nt	Go to original record
Description:	Declieuxia juniperina voucher Anderson 35443 (K) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Declieuxia juniperina
Original size:	1187 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	284	863	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcagg gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaacaa aggttcataa
151   agtgaaaagg ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcggtagtag agaagtcttt ccatcaaaaa ttcacaataa
251   gtataaattc aataactata actattaagc ctgaagtgaa ggataaacct
301   atatacatac gtagtgaaaa tgaatctatg ttttttctat gaaaaaacaa
351   agaattgatg tgaatagatt ccaagttgaa gaaaaatcat taatcaaacg
401   attcactcca tagtctgata gatcttttca agaattgatt aatctcaaga
451   attgattaat cggacgagaa taaagataga gtcccattct acatgtcaat
501   gctggcaaaa atgaaattta tagttaagag gaaaatccgt cgacttttaa
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details