JRC GMO-Amplicons | EURL GMFF

Record ID:	MH569081.1
Database:	nt	Go to original record
Description:	Festuca ovina plastid, complete genome.
Taxon:	Festuca ovina
Original size:	133569 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45518	46124	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg aggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaattaatta cgttgtgttg ttagtggaat tccttctaca
251   ttttagaaag aagggcttta tacacctaat aaacacgtat agatactgac
301   gattaatcac ggaacccata ttataatata ggttctttat tcttttttag
351   aatgaaataa atagaaatga ttatgaaata aaaaatttag aattttttgt
401   agaattattt ggattccatt ccaattgaat atttagtaat caaatccttc
451   aattcaaagt tttgaggtct ttaaaacagt ggattaatcg gacgaggaca
501   aagagagagt cccattctac atgtcaatac tgacaacaat gaaatttcta
551   gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details