JRC GMO-Amplicons | EURL GMFF

Record ID:	MH423068.1
Database:	nt	Go to original record
Description:	Dysosma versipellis chloroplast, complete genome.
Taxon:	Dysosma versipellis
Original size:	156583 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49047	49594	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactgag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcgg aaaagggata ggtgcagaga
151   ctcgatggaa gctgttctaa cgaatgaagt tggtcgaaga aaatctttct
201   attaaaactc cagaaaggat gaacctataa cctatatatg atatataggt
251   atacgtactg aaataaaata gaaaagatta atgaaatacg aatcctttat
301   ttaacttata caaaaattga aaaatttttc tgaatcgatt ccatgtggaa
351   gtaaggatcg aatattcact gatcaaatca gccactttag agtatagtat
401   gatatagatt ttgtgaagaa ctaactaatt ggacgagaat aaagatagag
451   tcccattcta catgtcaata ccgacaacaa tgaaatttat agtaagagga
501   aaatccgtcg actttagcaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details