JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115386.1
Database:	nt	Go to original record
Description:	Tiquilia atacamensis tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Tiquilia atacamensis
Original size:	588 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	588	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa aaaaaaaggt
151   tcagaaagcg aaaaaaagga taggtgcaga gactcgatgg aagctgttct
201   aacaaatgga gttgactgcc ttggtcgagg aatctttcca tcgaaacttc
251   cgaaaggatg gaggagaaac gtatatacat atatattcaa tactctatca
301   aatgaaatga ttaatgatga ccctaatctg gtttttttta tgttatgaaa
351   aaaaaaaaaa aaaaaaaaga gttggtctga cttgattcca cattgaaaaa
401   agggtcaaat attcattgat caaatcattc actccatagt ctgatagatc
451   ttttgaaaaa ctgattaatc ggacgagaat aaagatagag tcccgttcta
501   catgtcaata ccggcaaaaa tgaaatttat agtaaaagga aaatccgtcg
551   atttaaaaca tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details