JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115375.1
Database:	nt	Go to original record
Description:	Phacelia pinnatifida tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Phacelia pinnatifida
Original size:	574 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	574	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa agaaaggttc
151   agaaagcgaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtcgaggaat ctttcgatcg aaacttcaga
251   aaggatgaag gataaacgta tatacatatg tattcaatac tctatcaaat
301   gattaataac gaccctaatc tgttctgttt tttttttttc ataaaaaaaa
351   ggaagagttg gtatgaatcg attccacatt gcagaaagaa tcgaatattc
401   attgatcaaa tcattcactc catagtctga tagatctttt gaagaactga
451   ttaatcggac cagaataaag atagagtccc gttctacatg tcaataccgg
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt gaaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details