JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115369.1
Database:	nt	Go to original record
Description:	Nama dichotoma tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Nama dichotoma
Original size:	557 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	557	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcccgtttt ccgaaaacaa agaaaggttc
151   aaagggatag gtgcagagac tcaatggaag ctgttctaac aaatggagtt
201   gactgcgttg gtcgaggact ccttccatcg aaacttcaga aaggataaag
251   gagaaacgta tatacatatg tattcaatac tctatcaaat gaaatgatta
301   atgaaaatcc taatcttttt tttatgaaaa aaggaaagag ttggtgtgaa
351   tcgattccac attgaagaaa gaatcgaata ttcattgatc aaatcattca
401   ctccatagtc tgatagatcg tttgaagaac tgattaatcg gacgagaata
451   aagatagagt cccgttctac atgtcaatac cggcaacaat gaaatttatg
501   gtaagaggaa aatccgtcga ttttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details