JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115357.1
Database:	nt	Go to original record
Description:	Huidobria fruticosa tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Huidobria fruticosa
Original size:	593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	593	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgatcacttt caaattcaga gaaaccctgg aattaatcaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aagaaaaaaa aaaggatagg tgcagagact caatggaagc
201   tattctaaca aatggagttg actgcgttgc gttgttagag gaatcctttc
251   agccaaactt cagaaaggat gaagataaac ctatatatat acgtatacgt
301   actgaaatat tatatcaaat gattaatgac gacccgaatc tgtatctcta
351   tttttttata tgaaaaaata gaagaattgt tgtgaatcga ttcaaaattg
401   aagaaagaat cgaatattca ttgatcaaat cattcactcc atagtctgat
451   agatcttttg aagaactgat taatcggacg agaataaaga tagagtccca
501   ttccacatgt caataccgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt agaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details