JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115354.1
Database:	nt	Go to original record
Description:	Fagonia chilensis tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Fagonia chilensis
Original size:	553 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	553	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaatag gattgagcct tggtatggaa
51    acatactaag tgatcacttt caaattcaga gaaaccctgg aattagaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaaaaaaa agtcaattaa
151   aagaatcaaa tcaaaaaagt aaaaaaaaaa aaggataggt gcagagactc
201   aatggaagct gttctaaaaa atggagttga ctgctgcatt acgttagtca
251   agtcaaggaa tccttgcatc gaaacttttt caaggataaa ggataacctt
301   tctttctatc aaactctaaa aataaaaaat ataaatactt tttgaatcaa
351   ttggaagttg aagaaagaat caaatatgat tttctcaaat catttactcc
401   aaagtctgat atatcttttt tcaaaaagat tagtcagacg agaataaaga
451   tagagtccca ttctatatgt caatattgac aacaatgaaa tttatagtga
501   gaggaaaatc cgtcgatttt atcaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details