JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115344.1
Database:	nt	Go to original record
Description:	Chuquiraga atacamensis tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Chuquiraga atacamensis
Original size:	525 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	525	+	525 bp

Amplicon:

525 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaagtgaa
151   aataaaaaag gataggtgca gagactcgat ggaagctgtt ctaacgaatg
201   gagtggattg tcttgcgtta gtagaagaat ccttctatcg aaacttcaga
251   aaagatgaag gataaacctg taacataata cagaagaatt gttgtgaatc
301   gattccatat tgaagaaaga atagaatatt cattgataaa atcattcact
351   ccatgataaa atcattcact ccataatctg atagatcttt tgaagaactg
401   attaatcgga cgagaataaa gatagagtcc cgttctacat gtcaataccg
451   acaacaatga aatttttagt aagaggaaaa tccgtcgatt taaaaaatcg
501   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	525	506	2.36e-08

Match details