JRC GMO-Amplicons | EURL GMFF

Record ID:	MH115343.1
Database:	nt	Go to original record
Description:	Chorizanthe commissuralis tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Chorizanthe commissuralis
Original size:	614 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	614	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttaattg gttgagcctt agtatggaaa
51    cctactaagt gagaactttc aaaatcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaac tcctgctttc caaactttcc aaaagggaga
151   agaaataaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagttgactg tcttgcgttg ttataagaat tcttccaccg aaactcaaaa
251   aaggatgaag aataaaccta tatgcatacg tactttcgta ctttaatttc
301   aattactaga aatagaaaat aaaaacgaat aatgaattct gtattttatt
351   aatatgcaaa aaaatatgaa aaatagaaga attgtcgtga atttattcca
401   agtttaagaa agaatcgaat attcgtttat tttctattaa aaagattaaa
451   gcatttactc cacagtctga tagatctttt gaagaactga tcaatcggat
501   gagaatgaag atagagtccc attctacgtg tcaataccga caacaatgca
551   atttagagta agaggaaaat ccgtcgactt tataaatcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details