JRC GMO-Amplicons | EURL GMFF

Record ID:	MH036279.1
Database:	nt	Go to original record
Description:	Eriachne stipacea chloroplast, complete genome.
Taxon:	Eriachne stipacea
Original size:	134770 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46602	47207	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccacttttga taaacaagcg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtaattacg ttgtgttagt agggaaactc cctctaaatt
251   agagaaagaa gggctttata gatctaatac acacgtatgg atactggcat
301   agcaaaccaa aggattaatc acagaatcct atatcataat atcataatat
351   aggttcttta tttatttttg aaaatgaaat tcagaattat tatgaaatag
401   aaaattctga atccattcta ctcgaacatt gagtaatcaa atccttcatt
451   cattgttttt gagatctttt aaaaagtgga gattaatcgg acgaggataa
501   agagagagtc ccattctaca tgtcaatact gacaacaatg aaatttctag
551   taaaaggaaa atccgtcgac tttataagtt gtgagggttc aagtccctct
601   atcccc

View other 11 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details