JRC GMO-Amplicons | EURL GMFF

Record ID:	MH036255.1
Database:	nt	Go to original record
Description:	Eriachne melicacea chloroplast, complete genome.
Taxon:	Eriachne melicacea
Original size:	134867 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46661	47262	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccacttttga aaaataagcg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtaattacg ttgtgttggt agcaaaacaa aactccctct
251   aaattagagg aagaagggct ttatacatct aatacacacg tatggatact
301   ggcatagcaa accaaaggat taatcacaga atcctatatc ataatatagg
351   ttctttattt atttttgaaa atgaaattcg gaattattat gaaatagaaa
401   attctgaatc cattctactc gaacattgag taatcaaatc ctccaattca
451   ttgtttttga gatcttttca aaagtggatt aatcggacga ggataaagag
501   agagtcccat tctacatgtc aatactgaca acaatgaaat ttctagtaaa
551   aggaaaatcc gtcgacttta taagttgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details