JRC GMO-Amplicons | EURL GMFF

Record ID:	MH036239.1
Database:	nt	Go to original record
Description:	Eriachne avenacea chloroplast, complete genome.
Taxon:	Eriachne avenacea
Original size:	134727 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46577	47173	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccacttttga aaaataagcg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtaattacg ttgtgttggt agcaaaactc cctctaaatt
251   agagaaagaa gggctttata catctaatac acacgtatgg atactggcat
301   agcaaaccaa aggattaatc acagaatcct atatcataat ataggttctt
351   tatttatttt tgaaaatgaa attcggaatt attatgaaat agaaaattct
401   gaatccattc tactcgaaca ttgagtaatc aaatcctcca attcattgtt
451   tttgagatct tttcaaaagt ggattaatcg gacgaggata aagagagagt
501   cccattctac atgtcaatac tgacaacaat gaaatttcta gtaaaaggaa
551   aatccgtcga ctttataagt tgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details