JRC GMO-Amplicons | EURL GMFF

Record ID:	MH286325.1
Database:	nt	Go to original record
Description:	Pharnaceum aurantium tRNA-Ser (trnS-GCU) and tRNA-Ser (trnS-GGA) genes, complete sequence; ribosomal protein S4 (rps4) gene, complete cds; and tRNA-Thr (trnT-UGU), tRNA-Leu (trnL-UAA), and tRNA-Phe (trnF-GAA) genes, complete sequence; chloroplast.
Taxon:	Pharnaceum aurantium
Original size:	6278 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2728	3320	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaatta gcttgagcct tagtatggaa
51    acctactaag tgaaaccttc caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa ctcccttttt gcttttcaaa agcataaaga
151   agagttccga aagcaagaag aaaaaaggaa aggtgcagag actcaaagga
201   aggtgttcta acaaatgggg tcgacgggta gactttactg tcttgttatc
251   aggattcttc catcaaaact cccatccaaa gcttttttga ttttcgaaag
301   tcgaatagga aatagatatt cgagaatttt tcgaatagca aattctctct
351   aaatttcgac tcgaaagggc aaaaatttct atgctatgat aggtggaaga
401   atttttgggg atcaatgttc ctcgttaaca aaagaatcat tcacccatag
451   tctggctagt agatcttttc gaaaactacg agaatgaaga tagagtccgg
501   ttatacatgt caatactgac aacaaggaaa ttgaaagtaa gaggaaaatc
551   cgtcgacttt caaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details