JRC GMO-Amplicons | EURL GMFF

Record ID:	MH286351.1
Database:	nt	Go to original record
Description:	Hypertelis spergulacea tRNA-Ser (trnS-GGA) gene, complete sequence; ribosomal protein S4 (rps4) gene, complete cds; tRNA-Thr (trnT-UGU), tRNA-Leu (trnL-UAA), tRNA-Phe (trnF-GAA), tRNA-Glu (trnE-UUC), tRNA-Tyr (trnY-GUA), and tRNA-Asp (trnD-GUC) genes, complete sequence; photosystem II protein M (psbM) and cytochrome b6/f complex subunit VIII (petN) genes, complete cds; and tRNA-Cys (trnC-GCA) gene, complete sequence; chloroplast.
Taxon:	Hypertelis spergulacea
Original size:	12475 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3577	4163	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaatta gcttgagcct tagtatggaa
51    acctactaag tgaaaacttc caaattcaga gaaaccctgg aattaaaaaa
101   aaggggcaat cctgagccaa ctcctttttt ttcaaaagca aggaagggtt
151   cccaaagcaa aacaaaaaaa ggagaggtgc agagactcaa aggaagttgt
201   tctaacaaat ggtgtgtgcc gtcttgttct aagaaatctt ccatcaaaac
251   gcccatccaa agaggtttct tttgtgtttt tttttgcttt tcgaaaatcg
301   aatcgtaaag acatattcga gaaattttcg aatagaaact tctatctcaa
351   attggaatcg gaaaggcaaa aatgtcactt tctgtgatag gcggaagaat
401   ttgtgtgaat ctattccaag ttgccaaaag aatgattcac tcaatggtct
451   gtctaggaga tcgtttctag aactatgaga atgaagatag agtcccgttg
501   tacatgtcaa tcctgacaac aaggaaattg atagtaagag gaaaatccgt
551   cgattcaatt cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details