JRC GMO-Amplicons | EURL GMFF

Record ID:	MH286338.1
Database:	nt	Go to original record
Description:	Sarcobatus vermiculatus voucher M. J. Moore et al. 813 (OC) chloroplast, complete genome.
Taxon:	Sarcobatus vermiculatus
Original size:	152066 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46543	47130	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgaaaatttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt caaaaaaaaa aagattcaga
151   aagtaagaaa aaagaaaagg ataggtgcag agactcaaag gaagctgttc
201   taacaaatgg ggttgactgt gttgttataa gaattctttc atcaaaattc
251   taatccaaaa aaaggatata cgtactgaac tatatcaaat tattaaaaac
301   aacccgaatt tatttttata aatatgaaaa ttgatttatg aatctgaaag
351   aaatgaaaaa atttttatac gaagaagaat ttatgtgaat cgattccaag
401   ttgaaaaacg aatcaaattt taaaatcatt cactccagag tctgatagtt
451   ttttgaagaa ctaattaatc ggacgagaat gaagatagag tcccgttcta
501   catgtcaaga ctgacaacaa ggaaatttat aataagaaga aaatccgtcg
551   actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details