JRC GMO-Amplicons | EURL GMFF

Record ID:	MH286320.1
Database:	nt	Go to original record
Description:	Limeum aethiopicum chloroplast, partial genome.
Taxon:	Limeum aethiopicum
Original size:	133202 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49944	50495	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aagtaataaa
101   atatgggcaa tcctgagcca actcctttat ttatctcctt tatttatttt
151   aaaatttcaa acgaaaaaaa aaaaggatag gtgcagagac tcaaaggaag
201   ttgttctaac aaatggggtt gactgtgctg tgttgttata agaattcttc
251   catcgaaacc ccaattcaaa aaaaaaaaag gggtgatgcc ttatacttat
301   atatatatta tatacttata tatattatat atatatcaaa aagatggtat
351   atcaaaaaga tggacaatca aatattcatt catcaaatca ttcactccat
401   agtctgatag atcttttgaa aaacggatta attcggatga gaatgaagat
451   agagtcccgt tctacgtgtc aatactgaca acaatgaaat ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details