JRC GMO-Amplicons | EURL GMFF

Record ID:	MH286310.1
Database:	nt	Go to original record
Description:	Guapira discolor chloroplast, partial genome.
Taxon:	Guapira discolor
Original size:	153813 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47572	48158	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgcaaatttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt cttttgaaaa gaaaaaaaaa
151   ataagggttc agaaagcaag aaaaaaagga taggtgcaga gactcaaagg
201   aagctgttct aacaaatggg gttgactgtg ctgtgttgtt ataatcaaaa
251   ttccaatcaa aaaaaaaggg tgaagcatat acgtactgaa ctatatcaaa
301   tgattaataa caactttata tatttatgaa atgaaaaaat ttatatgaga
351   aaaaaattga agaattcttg tgattgtgaa tcgattccaa gttgaaaaaa
401   gaatcaaata ttcattcatc aaaccattca ctccatagtc tgatagattt
451   tttgaagaac tgattaatcg gacgagaatg aagagagagt cccgttctac
501   atgtcaatac tgacaacaat gaaatttata ataagaagaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details