JRC GMO-Amplicons | EURL GMFF

Record ID:	MH627219.1
Database:	nt	Go to original record
Description:	Cerastium arvense chloroplast, partial genome.
Taxon:	Cerastium arvense
Original size:	148092 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45133	45726	+	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttaatag gtttgagcct tagtatggaa
51    acctgctaag tgaaaacttt caaattcaga gaaaccctgg aaaaaaaagg
101   gcaatcctga gccaactcct taattgtctc ctaaaaaaaa cggattcaaa
151   aagaaagaat aaaaaaggat aggtgcagag actcaaagga agctgttcta
201   aaaaaaatgg ggacgtcctg aaatgattaa tgacaacccg aatttgttct
251   gtattttttt ttacttaatt atatatttat attataatta attaataata
301   atataatctc tagaaatcca tattatagga tagatagatt taaaatatgg
351   aaaaatgcaa gaattgttat ggatcgattc taagttgaaa gccgaataaa
401   tctttgagtt attcataaaa acattcatac taaccccata catagtctga
451   aagatctttt gaagaaggga ttaatcggat gagaataaag atagagtccc
501   gttctacatg tcaatattga caacaattaa atttatagta agaggaaaat
551   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details