JRC GMO-Amplicons | EURL GMFF

Record ID:	MK397927.1
Database:	nt	Go to original record
Description:	Phemeranthus sp. GY-2019 plastid, partial genome.
Taxon:	Phemeranthus sp. GY-2019
Original size:	158209 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49234	49831	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt ttttcaaaaa aaaaaaaaag
151   caaaaaataa gggttcaaaa agcaagaata aaaaaaaaaa aggagaggtg
201   cagagactca aaggaagctg ttctaacaaa tggggttgac tgtgctgtgt
251   tgttataaga attcttctat caaaactcca atccaaaaag ggtaaagcat
301   atacgtactg aactatatta cccgaatccg tatttttttt tagataattc
351   tatctaaaaa tagaatatga aatgaaaaaa tggaagaatt tttgtgttgt
401   gaatcgattc caagttgaaa aaagaatcaa atattcattt actccatagt
451   ctgatagatc ttttgaataa ctgattaatc ggacgagaat gaagatagag
501   tcccgttcta catgtcaata ctgacaaaaa tgaaatttat agtaagagga
551   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details