JRC GMO-Amplicons | EURL GMFF

Record ID:	MK397916.1
Database:	nt	Go to original record
Description:	Barbeuia madagascariensis voucher Ravelonarivo 3380 (MO) plastid, complete genome.
Taxon:	Barbeuia madagascariensis
Original size:	132939 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50583	51198	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt caaaagcaaa aaaaaagaag
151   ggttcagaaa gcaagaataa aaaaaaggat aggtgcagag actcaaagga
201   agctgttcta acaaatgggg ttgactgtgc tgtgttgtta taagaactct
251   tccatcaaaa ctccaatcca aaaagggtga agcatatatg tactgaacta
301   tatcaaatga ttaatgacaa cccgaatctg tatttttttt ctaattttta
351   tatatttatg aatatgaaat gaaaaaattt atatgaaaaa atggaagaat
401   ttttgtaaat cgattccaag ttgaaaaaag aatcaaatat tcatttatca
451   aatcattcac tccatagtct gatagatctt ttgaagaact gattaatcgg
501   acgagaatga agatagagtc ccgttctaca tgtcaatact gacaacaatg
551   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details