JRC GMO-Amplicons | EURL GMFF

Record ID:	MK397903.1
Database:	nt	Go to original record
Description:	Tetragonia tetragonioides voucher 13CS7950 (KUN) plastid, complete genome.
Taxon:	Tetragonia tetragonoides
Original size:	153258 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47122	47731	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aatgggcaat cctgagccaa ctcctttttt caaaagcaaa aaaagaaggt
151   tttagaaagc aagaaaaaaa ggataggtgc agagactcaa aggaagctat
201   tctaacaaat ggggttgact gtattgttat aagaattcgt ccatcaaaac
251   tccaatcaaa aataaagggg gggggtgaag catatacgta ctgaactatg
301   tcaaatgatt aatgacaacc cgaatctttc tttttttttt ctaattttta
351   tgaatatgaa ataaaaaatt ttatatgata aaatgtgaag aattattgtg
401   aatcgattcc aagttgaaaa aatgaatcaa atattcattc atcaaatcat
451   tcactccata gtctgataga tcttttgaag aactgattaa ttggacgaga
501   atgaagatag agtcccgttc tacatgtcaa tactgacaac aatgaaattt
551   atagtaagag gaaaatccgt cgacttttaa aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details