JRC GMO-Amplicons | EURL GMFF

Record ID:	MK397882.1
Database:	nt	Go to original record
Description:	Oxyria sinensis voucher 14CS9034 (KUN) plastid, complete genome.
Taxon:	Oxyria sinensis
Original size:	156034 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48905	49505	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaacccggg aataaaaaaa
101   tgggcaatcc tgagccaact ccttctttcc atttccaaaa ggaagaataa
151   aaaaggatag gtgcagagac tcaacggaag ctgttctaac aaacaaatgg
201   acgatcttgc gttattataa aaattcttcc atcgaaactc taaaaaggat
251   gaagaataaa cctatatgca tatgcagacg tagtgaaata taaatactat
301   atattacata aaaaaattta atttttaaat attgataacg acccgcattt
351   ttttttatga ctctgaaaaa aaggaagaat tgttgcgaag cgattccaag
401   ttgaagaaag aatcgaatac tcgtttaata aattaaagca tttattcccc
451   ggtctgatag atcttttgaa gaaccgatcc atcgtatgag aatgaagata
501   gagtcccatt ctacgtgtca atcctgacaa caatgaaatt tatagtaaga
551   ggaaaatccg tcgactttat aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details