JRC GMO-Amplicons | EURL GMFF

Record ID:	MH757117.1
Database:	nt	Go to original record
Description:	Haberlea rhodopensis mitochondrion, complete genome.
Taxon:	Haberlea rhodopensis
Original size:	484138 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	105091	105645	-	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa gggttcagag
151   agcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatcgt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaaatg atatataatg
301   gcgcgaatct gggtttttgt atatgaaaaa tggaagaatt ggtgtgaatt
351   gatttcacat tgatgaaaga atcgaatatt cattgatcaa atcattcact
401   ccatagtccg atagatcttt taaagaactt attaatcgga cgagaataaa
451   gatagagtcc cgttctacat gtcaataccg gcaacaatga aacttatagt
501   aagaggaaaa tccgtcgact ttcaaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details