JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181231.1
Database:	nt	Go to original record
Description:	Andropogon mannii isolate TK486 chloroplast, complete genome.
Taxon:	Andropogon mannii
Original size:	139015 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47326	47856	+	531 bp

Amplicon:

531 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataattat
251   aatataggtt ctttatttta tttttagaat gcaatttgga aggattatga
301   aatagaaaat tcataatttt tttagaatta ttgtgaatct attccaatcg
351   aatattgagt aatcaaatcc ttcaattcat tgttttcgag atcttttaaa
401   aagtggatta atcggacgag gataaagaga gagtcccatt ctacatgtca
451   atactgacaa caatgaaatt tctagtaaaa ggaaaatccg tcgactttat
501   aagtcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	531	512	2.39e-08

Match details