JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181224.1
Database:	nt	Go to original record
Description:	Andropogon canaliculatus isolate TK488 chloroplast, complete genome.
Taxon:	Andropogon canaliculatus
Original size:	138817 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47837	48370	+	534 bp

Amplicon:

534 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttttg aaaaaacaag cggttctcaa
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaagtaatag cgattaatca cagaacccat attataatat
251   aggttcttta ttttattttt agaatgaaat aatgaaatta ggaaggatta
301   tgaaatagaa aattcataat ttttttagaa ttattgtgaa tctattccaa
351   tcgaatattg agtaatcaaa tccttcaatt cattgttttc gagatcttta
401   aaaaagtgga ttaatcggac gaggataaag agagagtccc attctacatg
451   tcaatactga caacaatgaa atttctagta aaaggaaaat ccgtcgactt
501   tataagtcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	534	515	2.40e-08

Match details