JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181205.1
Database:	nt	Go to original record
Description:	Schizachyrium cubense isolate TK755 chloroplast, complete genome.
Taxon:	Schizachyrium cubense
Original size:	139130 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48196	48725	+	530 bp

Amplicon:

530 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat tttattttta gaatgaaatt aggaaggatt atgaaataga
301   aaattcataa tttttttaga attattgtga atctattcca atcgaatatt
351   gagtaatcaa atccttcaat tcattgtttt cgagatcttt tcttttaaaa
401   agtggattaa tcggacgagg ataaagagag agtcccattc tacatgtcaa
451   tactgacaac aatgaaattt ctagtaaaag gaaaatccgt cgactttata
501   agtcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	530	511	2.38e-08

Match details