JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181201.1
Database:	nt	Go to original record
Description:	Schizachyrium lomaense isolate TK768 chloroplast, complete genome.
Taxon:	Schizachyrium lomaense
Original size:	138660 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48385	48923	+	539 bp

Amplicon:

539 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttttt ttgaaaaaac aggcggttct
151   caaactagaa cccaaaggaa aaggataggt gcagagactc aatggaagct
201   gttctaacga atcgaagtaa taacgattaa tcacagaacc catattataa
251   tataggttct ttatttgatt ttgagaatga aattaggaag gattatgaaa
301   tagaaaattc agaatttttt tagaattatt gtgaatctat tccaatcgaa
351   tattgagtaa tcaaatcctt caattcattg ttttcgagat cttttaaaaa
401   gtggattaat cggacgagga taaagagaga gtcccattct acatgtcaat
451   actgatcaat actgacaaca atgaaatttc tagtaaaagg aaaatccgtc
501   gactttataa gtcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.42e-08
primerB	100.0	20 bp	0	0	539	520	2.42e-08

Match details