JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181199.1
Database:	nt	Go to original record
Description:	Hyparrhenia anemopaegma isolate TK745 chloroplast, complete genome.
Taxon:	Hyparrhenia anemopaegma
Original size:	138502 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46959	47491	+	533 bp

Amplicon:

533 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   taatataggt tctttatttt attttgagaa tgaaatttgg aaggattatg
301   aagaggtata gaaaattcat aattttttta gaattattgt gaatctattc
351   caatcgaata ttgagtaatc aaatccttca attcattgtt ttcgagatct
401   tttagtggat taatcggacg aggataaaga gagagtccca ttctacatgt
451   caatactgac aacaatgaaa tttctagtaa aaggaaaatc cgtcgacttt
501   ataagtcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	533	514	2.40e-08

Match details