JRC GMO-Amplicons | EURL GMFF

Record ID:	MH181181.1
Database:	nt	Go to original record
Description:	Andropogon chrysostachyus isolate TK482 chloroplast, complete genome.
Taxon:	Andropogon chrysostachyus
Original size:	139459 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47795	48323	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat ttttttttag aatgaaattt ggaaggatta tgaagaggta
301   tagaaaattc ataatttttt tagaattatt gtgaatctat tccaatcgaa
351   tattgagtaa tcaaatcctt caattcattg ttttcgagat cttttaaaaa
401   gtggattaat cggacgagga taaagagaga gtcccattct acatgtcaat
451   actgacaaca atgaaatttc tagtaaaagg aaaatccgtc gactttataa
501   gtcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details