JRC GMO-Amplicons | EURL GMFF

Record ID:	MG792012.1
Database:	nt	Go to original record
Description:	Burmannia disticha chloroplast, complete genome.
Taxon:	Burmannia disticha
Original size:	157480 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	44660	45245	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaacccctgg aattaaaaat
101   gggcaatcct gagccaaatc tttatttgat ttgagatata gaaaattaga
151   atccaaatca aaaaagtata ggtgcagaga ctcaatggaa gttgttctaa
201   caaatcaaat gaaatgaaat tgactatgtt acgttggaag aatgaatctc
251   tcggtcgaaa tttaattacg cttatggaaa ggaaaaacat ataaaaacat
301   attaacatat atatatatat attataaata taatcatatt tagaattatt
351   gtattgtgga tctattccaa tagaaattga agtaagactt gaatatacaa
401   ctattaaatc attcattaca gattacagaa tctaatagat cgttttgaaa
451   aaccgattaa gattaatcgg attagaataa agagagagtc ccattctaca
501   tgtcaatatc gacaacaatg aaatttctag taagaggaaa atccgtcgac
551   tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details