JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235015.1
Database:	nt	Go to original record
Description:	Cissus repanda chloroplast DNA, trnL-trnF IGS, isolate: 7396, partial sequence.
Taxon:	Cissus repanda
Original size:	1019 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	582	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ccgttttccg aaaacgacca agggttcaga
151   aaacgataat aaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaaaaacaa atggagttga ctatgttgcg ttgttaaacg aatccttcca
251   tcaaaactcc agaaccagaa aggatgaagg ataaacctat atacataggt
301   actgaaatat tatatcaaat gattaatgat ggctcgaatc tgtatttttt
351   gatatgaaaa atgaaagaat tgttgtaaat agattacaaa gaaaagaatc
401   gaatattcat tgctcaaatc attcactcca tagtccgata aatcttttga
451   agaactgatt aatcggacga gaataaagat agagtcccat tctacatgtc
501   aataccgaca agaatgaaat ttatagtaag aggaaaatcc gtcgacttta
551   aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details