JRC GMO-Amplicons | EURL GMFF

Record ID:	AB235093.1
Database:	nt	Go to original record
Description:	Leea indica chloroplast DNA, trnL-trnF IGS, isolate: 7458, partial sequence.
Taxon:	Leea indica
Original size:	1019 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	581	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aattacaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga agggttcaga
151   aaacgataat gaaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgt ggtgcgttgt taaaggaatc cttgcatcaa
251   aactccagaa aggatgaagg ataaacgtat atacatacgt acagaaatat
301   tatatcaaat gattaatgac agctcgaatc tgtgttttgt gatatgaaaa
351   atgaaagaat tgttgtaaat cgattccaag ttgaagaaag aaaagaatcg
401   aatattcatt gatcaaatca ttcactccat agtccgatag atcttttgaa
451   gaactgatta atcggacgag aataaagaga gagtcccatt ctacatgtca
501   ataccgacaa caatgaaatt tatagtaaga ggaaaatccg tcgactttaa
551   aaatcgnnnn ngttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details