JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ354001.1
Database:	nt	Go to original record
Description:	Poa margilicola trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa margilicola
Original size:	1820 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	837	1444	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcnn nnnnnnaacc
51    tgctaagtgg taacttccaa attcagagaa accctggaat taaaaaaggg
101   caatcctgag ccaaatccgt gttttgagaa aacaaggggg ttctcgaact
151   agaatacaaa ggaaaaggat aggtgcagag actcaatgga agctgttcta
201   acgaatcgag ttaattacgt tgtgttgtta gtggaattcc ttcgaaattc
251   taaaaagaag ggctttatac agctaataaa cacgtataga tactgagata
301   gcaaacgatt aatcacagaa cccatattat aatattggtt ctttattctt
351   ttttagaatg aaatagaaat gattatgaaa taaaaaattc ataatttttt
401   tagaattatt gtgaatccat tccaatcgaa tatttagtaa tcaaatcctt
451   caattcaaag ttttgaggtc gttaaaaaag tggattaatc ggatgaggac
501   aaagagagag tcccattcta catgtcaata ctgacaacaa tgaaatttct
551   agtaaaagga aaatccgtcg actttataag tcgtgagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details