JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ354000.1
Database:	nt	Go to original record
Description:	Poa palustris voucher Gillespie 6461 CAN trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Poa palustris
Original size:	1847 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	849	1458	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    cctgctaagt ggtaacttcc aaattcagag aaaccctgga attaaaaaag
101   ggcaatcctg agccaaatcc gtgttttgag aaaacaaggg ggttctcgaa
151   ctagaataca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttaattac gttgtgttgt tagtggaatt ccttcgaaat
251   tctaaaaaga agggctttat acagctaata aacacgtata gatactgaga
301   tagcaaacga ttaatcacag aacccatatt ataatattgg ttctttattc
351   ttttttagaa tgaaatagaa atgattatga aataaaaaat tcataatttt
401   tttagaatta ttgtgaatcc attccaatcg aatatttagt aatcaaatcc
451   ttcaattcaa agttttgagg tcgttaaaaa agtggattaa tcggatgagg
501   acaaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details